[Animal experiments, morphologic and endocrinologic studies following various coagulation technics].

CO2-pelviscopy or laparotomies were performed on 370 rabbits followed by coagulation of the distal uterine horns with unipolar, bipolar, endo-, or CO2-laser coagulation. As we could demonstrate by the following laparotomy, macroscopically with exact measuring of the destroyed region and later on by histological, histochemical and electronmicroscopic examinations, the extent of tissue destruction during coagulation of the distal uterine horns by endocoagulation or also bipolar high-frequency technique--when operated at lower coagulation currents--was significantly less compared to the destruction caused by monopolar high-frequency technique using monopolar high-frequency coagulation, extensive destruction of the uterine horns and the surrounding mesoalpingeal tissue occurred after a coagulation time of only 10 sec. With application of the bipolar coagulation technique the destruction of tissue was much more localized. - Rabbits distal uterine horns which were coagulated by CO2laser were examined by a relaparotomy 4-12 weeks later. Macroscopic, histological and enzymehistochemical studies showed that initial recanalization had occurred in 8 out of 23 cases. Utilizing the endocoagulation technique with a forceps temperature of 120 degrees C and a coagulation time of 20 sec, coagulation of the uterine horns occurred in all cases without complications. During the "Second-look" operation and the final tissue studies a complete division (of approximately 0.5 cm) of the uterine horns was evident, and it could be demonstrated, that the technique did not have any effect on the mesosalpingeal tissue. After stimulation of 49 rabbits with 50 I.U. of HCG i.v. the control group showed a clear rise in average progesterone levels of 12.69 +/- 6.02 ng/ml up to the 11th day of pseudo-pregnancy followed by gradual decrease in concentrations, returning to basal levels from day 14-25. The average progesterone levels of the two animal groups coagulated by using the monopolar high-frequency current were significantly lower than the control levels or the progesterone concentrations in the endocoagulation or bipolar group.