Dorland L, Haverkamp J, Viliegenthart J F, Strecker G, Michalski J C, Fournet B, Spik G, Montreuil J
Eur J Biochem. 1978 Jun 15;87(2):323-9. doi: 10.1111/j.1432-1033.1978.tb12381.x.
360-MHz proton nuclear magnetic resonance spectra were recorded of 10 sialyl-oligosaccharides isolated from urine of sialidosis patients. Their structures are related to the complex asparagine-linked glycan chains of glycoproteins. By correlation of these spectra and comparison with spectra of reference glycopeptides and sialyl-lactose isomers it was possible to assign all signals belonging to anomeric, mannose H-2, sialic acid H-3 and N-acetyl protons. The number of the consituting monosaccharide residues of the oligomers can be obtained by integration of the above-mentioned signals. The chemical shifts of the anomeric and mannose H-2 protons give information about the type of glycan structure (mono-, bi-, triantennary) and the presence of terminal sialic acid at each of the antennas. The chemical shifts of sialic acid H-3 protons are typical for sialic acid residues in 2 leads to 3 or 2 leads to 6 linkage to galactose.
记录了从唾液酸沉积症患者尿液中分离出的10种唾液酸寡糖的360兆赫质子核磁共振谱。它们的结构与糖蛋白中复杂的天冬酰胺连接聚糖链有关。通过这些光谱的关联以及与参考糖肽和唾液酸乳糖异构体光谱的比较，有可能确定所有属于异头物、甘露糖H-2、唾液酸H-3和N-乙酰基质子的信号。通过对上述信号的积分可以得到寡聚物中构成单糖残基的数量。异头物和甘露糖H-2质子的化学位移提供了有关聚糖结构类型（单天线、双天线、三天线）以及每个天线末端唾液酸存在情况的信息。唾液酸H-3质子的化学位移是唾液酸残基与半乳糖以2→3或2→6连接的典型特征。
