Sugiyama S, Takahashi M, Furuya S
J Ultrastruct Res. 1983 Nov;85(2):219-27. doi: 10.1016/s0022-5320(83)90108-9.
Broad bundles of actinlike microfilaments are found in the basal cells of the outer root sheath of the hair follicle. The filaments react specifically with heavy meromyosin to form a fuzzy structure, and disappear from the cells after the actin depolymerization treatment. An array of the filament bundles alters along with the cell shape changes of the basal cells. In the flattened cells of the hair follicle bulb, the bundles appear to run parallel to the long axis of the cells, and are present in the basal cytoplasm. In the cuboidal cells of the suprabulbar and the more superficial portion of the follicle, the bundles are perpendicular to the basal plasma membrane, and are disposed mainly in the basal cytoplasm. The microfilaments are associated with the basal plasma membrane directly or via insertion into the plaque of the membrane, where filamentous or amorphous materials link the membrane and the basal lamina. In vitro treatment of cytochalasin B for up to 8 hr causes no visible change in the distribution pattern of the bundles nor abnormal changes in the cell shape. Thus, the actinlike microfilament bundles appear to provide a cytoskeletal system responsible for the maintenance of the cell shape change, since they are disposed in the direction and area in which a maintenance force for the cell shape change may be required, and other cytoskeletal systems of the cells such as microtubules and tonofilament-desmosome complexes are poorly developed, and no hemidesmosomes are present in the cells.
在毛囊外根鞘的基底细胞中发现了宽束状的肌动蛋白样微丝。这些微丝与重酶解肌球蛋白发生特异性反应形成模糊结构，并在肌动蛋白解聚处理后从细胞中消失。微丝束的排列随着基底细胞形状的变化而改变。在毛囊球部扁平细胞中，微丝束似乎与细胞长轴平行，并存在于基底细胞质中。在球上部及毛囊更浅表部分的立方体细胞中，微丝束垂直于基底质膜，且主要分布于基底细胞质中。微丝直接或通过插入膜斑与基底质膜相连，在膜斑处丝状或无定形物质连接膜和基膜。用细胞松弛素B体外处理长达8小时，微丝束的分布模式未见明显变化，细胞形状也未出现异常改变。因此，肌动蛋白样微丝束似乎提供了一个负责维持细胞形状变化的细胞骨架系统，因为它们分布在可能需要维持细胞形状变化的力的方向和区域，而且细胞的其他细胞骨架系统如微管和张力丝-桥粒复合体发育不良，细胞中也不存在半桥粒。
