Land H, Grez M, Ruppert S, Schmale H, Rehbein M, Richter D, Schütz G
Nature. 1983;302(5906):342-4. doi: 10.1038/302342a0.
The nonapeptide hormone oxytocin-like arginine-vasopressin (AVP) is synthesized as part of a larger precursor polypeptide. The precursor also includes the neurophysin molecule with which the hormone is associated in the neurosecretory granules of the hypothalamo-pituitary tract. A protein of molecular weight (Mr) approximately 20,000 has been isolated from supraoptic nuclei of rat hypothalami which, after tryptic cleavage, released a neurophysin-like molecule of Mr approximately 10,000 and an oligopeptide related to oxytocin. This result was complemented by in vitro translation of bovine hypothalamic mRNA. Among the primary translation products a single polypeptide of Mr approximately 16,500 was shown to contain antigenic determinants recognized by specific antisera against bovine neurophysin I and oxytocin. Here we report the amino acid sequence of the bovine oxytocin-neurophysin I (OT-NpI) precursor which was derived from sequence analysis of the cloned cDNA. As is the case for the bovine arginine-vasopressin-neurophysin II (AVP-NpII) precursor, the signal sequence of the OT-NpI precursor is immediately followed by the nonapeptide hormone which is connected to neurophysin I by a Gly-Lys-Arg sequence. A striking feature of the nucleic acid sequence is the 197-nucleotide long perfect homology with the AVP-NpII precursor mRNA sequence encoding the conserved middle part of neurophysins I and II.
九肽激素催产素样精氨酸加压素（AVP）是作为一种更大的前体多肽的一部分合成的。该前体还包括神经垂体素分子，在丘脑下部 - 垂体束的神经分泌颗粒中，激素与该分子相关联。已从大鼠下丘脑的视上核中分离出一种分子量（Mr）约为20,000的蛋白质，经胰蛋白酶切割后，释放出一种Mr约为10,000的神经垂体素样分子和一种与催产素相关的寡肽。牛下丘脑mRNA的体外翻译补充了这一结果。在初级翻译产物中，一种Mr约为16,500的单一多肽显示含有针对牛神经垂体素I和催产素的特异性抗血清所识别的抗原决定簇。在此，我们报告了牛催产素 - 神经垂体素I（OT - NpI）前体的氨基酸序列，该序列来自克隆cDNA的序列分析。与牛精氨酸加压素 - 神经垂体素II（AVP - NpII）前体的情况一样，OT - NpI前体的信号序列紧接着九肽激素，该激素通过甘氨酸 - 赖氨酸 - 精氨酸序列与神经垂体素I相连。核酸序列的一个显著特征是与编码神经垂体素I和II保守中间部分的AVP - NpII前体mRNA序列具有197个核苷酸的完全同源性。
