Molecular cloning of complementary DNA: preparation of a plasmid vector with low transformation background.
作者信息
Leriche A, Christophe D, Brocas H, Vassart G
出版信息
Anal Biochem. 1983 Feb 15;129(1):249-52. doi: 10.1016/0003-2697(83)90077-5.
PMID:6687985
Abstract
A simple method that allows the rapid preparation of oligo dG-tailed plasmid vectors is presented. The procedure involves purification of the tailed molecules by hybridization to oligo dC-cellulose followed by a stepwise thermal elution. The resulting plasmid is virtually devoid of transformation activity in the absence of oligo dC-tailed DNA fragments. It allows construction of cDNA libraries with as low as 1% of colonies harboring wild-type plasmids.
摘要
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