Leriche A, Christophe D, Brocas H, Vassart G
Anal Biochem. 1983 Feb 15;129(1):249-52. doi: 10.1016/0003-2697(83)90077-5.
A simple method that allows the rapid preparation of oligo dG-tailed plasmid vectors is presented. The procedure involves purification of the tailed molecules by hybridization to oligo dC-cellulose followed by a stepwise thermal elution. The resulting plasmid is virtually devoid of transformation activity in the absence of oligo dC-tailed DNA fragments. It allows construction of cDNA libraries with as low as 1% of colonies harboring wild-type plasmids.
本文介绍了一种能够快速制备寡聚dG尾质粒载体的简单方法。该方法包括通过与寡聚dC-纤维素杂交来纯化带尾分子，随后进行逐步热洗脱。在没有寡聚dC尾DNA片段的情况下，所得质粒几乎没有转化活性。它能够构建cDNA文库，其中含有野生型质粒的菌落低至1%。
