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The nucleotide sequence of cDNA complementary to the C1 component of rat prostatic binding protein.

作者信息

Delaey B, Dirckx L, Peeters B, Volckaert G, Mous J, Heyns W, Rombauts W

出版信息

Eur J Biochem. 1983 Jul 1;133(3):645-9. doi: 10.1111/j.1432-1033.1983.tb07512.x.

Abstract

The mRNA for component C1 of rat prostatic binding protein has been cloned and characterized. A partially purified mRNA fraction for this complex protein was reverse-transcribed into double-stranded cDNA and cloned into the PstI site of plasmid pBR 322. The 426-base-pair insert of the recombinant plasmid pC1A75 was completely sequenced. The coding region corresponds precisely to the 88 amino acid residues of C1 and in addition contains the information of a signal peptide of 23 residues. The 5' non-coding region counts only 19 nucleotides and is incomplete but the 3'-terminal non-coding part of 60 nucleotides extends into the poly(A) tail. Sequence analysis of other C1-positive clones indicates the presence of sequence rearrangements which must have occurred during the cloning procedure. Possible mechanisms for the generation of these cloning artefacts are discussed.

摘要

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1
The nucleotide sequence of cDNA complementary to the C1 component of rat prostatic binding protein.
Eur J Biochem. 1983 Jul 1;133(3):645-9. doi: 10.1111/j.1432-1033.1983.tb07512.x.

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