有证据表明，蛋白酶的作用并非特异性地参与由商业牛血清白蛋白在培养中引起的兔胚泡孵化。

Evidence that protease action is not specifically involved in the hatching of rabbit blastocysts caused by commercial bovine serum albumin in culture.

作者信息

Kane M T

出版信息

J Reprod Fertil. 1983 Jul;68(2):471-5. doi: 10.1530/jrf.0.0680471.

DOI:10.1530/jrf.0.0680471

Abstract

Commercial samples of bovine serum albumin (BSA) in a complex medium caused growth of 1-cell rabbit embryos to completely hatched blastocysts. Heat treatment of the BSA at 65 or 80 degrees C significantly decreased blastocyst formation and expansion and destroyed the ability to cause blastocyst hatching. Addition of trypsin at levels down to 20 ng/ml caused the formation of hatched blastocysts which degenerated rapidly. The effects of 5 protease inhibitors (ovomucoid trypsin inhibitor, alpha-1-antitrypsin, TAME, TLCK and soybean) were tested. Ovomucoid trypsin inhibitor, TAME and TLCK significantly inhibited blastocyst hatching but only at the highest concentration used. These inhibitors also reduced blastocyst formation and expansion, indicating that their effect was not specifically on blastocyst hatching in vitro. It is concluded that hatching of rabbit blastocysts is probably not dependent on protease action.

摘要

在复合培养基中的商业牛血清白蛋白（BSA）样品可使单细胞兔胚胎发育至完全孵化的囊胚。将BSA在65或80摄氏度下进行热处理会显著降低囊胚的形成和扩张，并破坏其导致囊胚孵化的能力。添加低至20 ng/ml的胰蛋白酶会导致孵化囊胚的形成，但这些囊胚会迅速退化。测试了5种蛋白酶抑制剂（卵类粘蛋白胰蛋白酶抑制剂、α-1抗胰蛋白酶、对甲苯磺酰-L-精氨酸甲酯、N-对甲苯磺酰-L-赖氨酸氯甲基酮和大豆蛋白酶抑制剂）的效果。卵类粘蛋白胰蛋白酶抑制剂、对甲苯磺酰-L-精氨酸甲酯和N-对甲苯磺酰-L-赖氨酸氯甲基酮仅在使用的最高浓度下显著抑制囊胚孵化。这些抑制剂还减少了囊胚的形成和扩张，表明它们的作用并非特异性针对体外囊胚孵化。结论是兔囊胚的孵化可能不依赖于蛋白酶的作用。