Agata K, Yasuda K, Okada T S
Dev Biol. 1983 Nov;100(1):222-6. doi: 10.1016/0012-1606(83)90214-2.
The question of whether or not delta-crystallin gene (delta-gene) is transcribed in tissues of chicken embryos other than lens was examined by Northern blot analysis, using cloned delta-crystallin cDNA as a probe. delta-Crystallin mRNA was detected in neural retinas, brains, and limb buds of 3.5-day-old embryos. However, the distribution of delta-gene transcripts is limited to lens only in posthatched chickens. It was indicated that the ratio of large delta-crystallin RNA, probably precursor RNA, to mature delta-crystallin mRNA is always much higher in nonlens tissues.
使用克隆的δ-晶体蛋白cDNA作为探针，通过Northern印迹分析来检测δ-晶体蛋白基因（δ-基因）是否在鸡胚除晶状体之外的组织中被转录。在3.5日龄胚胎的神经视网膜、脑和肢芽中检测到了δ-晶体蛋白mRNA。然而，δ-基因转录本的分布在孵化后的鸡中仅限于晶状体。结果表明，在非晶状体组织中，大的δ-晶体蛋白RNA（可能是前体RNA）与成熟的δ-晶体蛋白mRNA的比例总是高得多。
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