Hejtmancik J F, Beebe D C, Ostrer H, Piatigorsky J
Dev Biol. 1985 May;109(1):72-81. doi: 10.1016/0012-1606(85)90347-1.
The levels of delta- and beta-crystallin mRNAs were examined by cDNA hybridization in the embryonic and posthatched chicken eye lens. Four different cloned beta-crystallin cDNAs were used, allowing discrimination among different members of the beta-crystallin family. Each crystallin mRNA displayed a characteristic temporal and spatial pattern in the developing lens. delta-Crystallin mRNA accumulated rapidly during early embryonic development; by contrast, the beta-crystallin mRNAs began to accumulate rapidly near the end of embryogenesis. Both delta- and beta-crystallin mRNAs increased in the lens for the first month after hatching and began to decrease 3 months after hatching. The levels of the delta- and the different beta-crystallin mRNAs were also differentially regulated in cultured embryonic lens epithelia. The most fiber cell specific crystallin gene product in the differentiating lens was the beta 35 mRNA. These experiments provide a quantitative basis for exploring the differential expression of the delta- and beta-crystallin gene families in the chicken lens.
通过cDNA杂交检测了胚胎期和孵化后鸡眼晶状体中δ-和β-晶状体蛋白mRNA的水平。使用了四种不同克隆的β-晶状体蛋白cDNA，从而能够区分β-晶状体蛋白家族的不同成员。每种晶状体蛋白mRNA在发育中的晶状体中都呈现出独特的时空模式。δ-晶状体蛋白mRNA在胚胎发育早期迅速积累；相比之下，β-晶状体蛋白mRNA在胚胎发育接近尾声时开始迅速积累。孵化后的第一个月，晶状体中δ-和β-晶状体蛋白mRNA的水平均升高，孵化3个月后开始下降。在培养的胚胎晶状体上皮细胞中，δ-和不同β-晶状体蛋白mRNA的水平也受到不同的调节。在分化的晶状体中，最具纤维细胞特异性的晶状体蛋白基因产物是β35 mRNA。这些实验为探索鸡晶状体中δ-和β-晶状体蛋白基因家族的差异表达提供了定量依据。
