Gilfillan A M, Hollingsworth M, Jones A W
Br J Pharmacol. 1983 Jun;79(2):363-71. doi: 10.1111/j.1476-5381.1983.tb11008.x.
Lung slices from adult rats incubated in [methyl-3H]-choline chloride formed [3H]-disaturated phosphatidylcholine ( [3H]-DSPC) which was used as an index of lung surfactant. The slices were perifused after 3 h incubation in [methyl-3H]-choline chloride and the overflow of [3H]-DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [3H]-DSPC rapidly declined over the first 30 min of perifusion and then declined slowly. Salbutamol induced a prolonged, and sometimes delayed, increase in [3H]-DSPC overflow, which was reduced by (+/-)-propranolol. Potassium chloride produced an immediate, and usually transient, increase in [3H]-DSPC overflow which was not modified by atropine or (+/-)-propranolol. Adenosine 5'-triphosphate, but not phenylephrine, also increased [3H]-DSPC overflow. This method can measure the magnitude and time-course of lung surfactant secretion induced by drugs.
在含有[甲基-³H]-氯化胆碱的培养液中孵育的成年大鼠肺切片,会形成[³H]-二饱和磷脂酰胆碱([³H]-DSPC),其用作肺表面活性剂的指标。在含有[甲基-³H]-氯化胆碱的培养液中孵育3小时后,对切片进行灌流,并将[³H]-DSPC的流出量作为速率系数,用作表面活性剂分泌的指标。在灌流的最初30分钟内,[³H]-DSPC的基础流出量迅速下降,然后缓慢下降。沙丁胺醇可导致[³H]-DSPC流出量长期增加,有时会延迟增加,而(±)-普萘洛尔可使其降低。氯化钾可使[³H]-DSPC流出量立即增加,通常是短暂增加,阿托品或(±)-普萘洛尔对此无影响。5'-三磷酸腺苷而非去氧肾上腺素也可增加[³H]-DSPC流出量。该方法可测量药物诱导的肺表面活性剂分泌的幅度和时间进程。