Massaro D, Clerch L, Massaro G D
J Appl Physiol Respir Environ Exerc Physiol. 1981 Sep;51(3):646-53. doi: 10.1152/jappl.1981.51.3.646.
We examined the effect of the ventilatory rate and the temperature of excised lungs and of increased body temperature of anesthetized spontaneously breathing rats on the centrifugal sedimentation of disaturated phosphatidylcholine (DSPC) present in lung lavage returns. More DSPC sedimented from lungs ventilated at low than at high rates, and sedimentation of DSPC and lung volume loss were temperature dependent, 41 greater than 37 greater than 4 degrees C. Most of the noncellular sedimented material was tubular and common myelin; these had diminished ability to lower surface tension rapidly compared with less-aggregated surfactant. More aggregated DSPC accumulated and lung volume decreased more in spontaneously breathing rats anesthetized for 30 min than in rats killed immediately after being anesthetized; these changes were greater after 30 min of anesthesia in hyperthermic rats (40.4 +/- 0.3 degrees C) than in normothermic rats (37.4 +/- 0.1 degrees C). These studies have shown a correlation between the increased accumulation of surfactant as large aggregates and the loss of alveolar stability; however, a cause and effect between these events has not yet been shown.
我们研究了通气速率、离体肺温度以及麻醉状态下自主呼吸大鼠体温升高对肺灌洗回收液中双饱和磷脂酰胆碱（DSPC）离心沉降的影响。与高通气速率相比，低通气速率时肺中沉降的DSPC更多，且DSPC的沉降和肺容积损失均与温度有关，41℃时大于37℃时大于4℃时。大多数非细胞沉降物质为管状和普通髓鞘样结构；与聚集程度较低的表面活性剂相比，这些物质快速降低表面张力的能力减弱。与麻醉后立即处死的大鼠相比，麻醉30分钟的自主呼吸大鼠中聚集的DSPC积累更多，肺容积减少更多；与正常体温大鼠（37.4±0.1℃）相比，高温大鼠（40.4±0.3℃）麻醉30分钟后这些变化更明显。这些研究表明，表面活性剂以大聚集体形式增加的积累与肺泡稳定性丧失之间存在相关性；然而，这些事件之间的因果关系尚未得到证实。
