Cestaro B, Cervato G, Ferrari S, Di Silvestro G, Monti D, Manitto P
Ital J Biochem. 1983 Sep-Oct;32(5):318-29.
Interaction of bilirubin with phospholipid bilayers was studied at physiological pH above and below the gel-liquid crystalline phase transition of small unilamellar vesicles of dipalmitoylphosphatidylcholine. Chromatographic, calorimetric and 1H-NMR evidences strongly suggest that dianion form of bilirubin binds to the polar heads of the phosphatidylcholines protruding from the outer leaflet of the vesicles, whilst acid bilirubin, which is insoluble in water, is hydrophobically inserted into the lipophilic region of the bilayers. The surface-bound bilirubin is promptly removed from vesicles, whilst the acid form hydrophobically inserted into the vesicles is firmly bound to the membrane in the gel state. This pool of bilirubin could perturb the chemico-physical properties of the membrane (i.e., fluidity, phase transition, etc. ...) thus contributing to perturbation of the biological properties of living cells.
在生理pH值下，研究了胆红素与二棕榈酰磷脂酰胆碱小单层囊泡凝胶-液晶相转变温度以上和以下的磷脂双层的相互作用。色谱、量热和1H-NMR证据有力地表明，胆红素的二价阴离子形式与从囊泡外小叶突出的磷脂酰胆碱的极性头部结合，而不溶于水的酸性胆红素则疏水插入双层的亲脂区域。表面结合的胆红素会迅速从囊泡中去除，而疏水插入囊泡的酸性形式则在凝胶状态下牢固地结合在膜上。这部分胆红素可能会扰乱膜的化学物理性质（即流动性、相变等……），从而导致活细胞生物学性质的紊乱。
