Tawa R, Kitoh M, Takeshima S, Hirose S
Nucleic Acids Symp Ser. 1983(12):107-10.
Immobilized xanthine oxidase (XO) was prepared by intermolecular cross-linking to controlled pore-glass using glutaraldehyde. The performance of enzyme reactors packed with the immobilized XO were considered theoretically and investigated experimentally. Using a post-column reactor packed with the immobilized XO, simultaneous determinations of hypoxanthine and xanthine by reversed-phase high-performance liquid chromatography were achieved.
通过使用戊二醛将黄嘌呤氧化酶（XO）分子间交联到可控孔径玻璃上来制备固定化黄嘌呤氧化酶。从理论上考虑并通过实验研究了填充有固定化XO的酶反应器的性能。使用填充有固定化XO的柱后反应器，通过反相高效液相色谱法实现了次黄嘌呤和黄嘌呤的同时测定。
