Immobilization of xanthine oxidase to controlled pore-glass. Application to high-performance liquid chromatography.

Immobilized xanthine oxidase (XO) was prepared by intermolecular cross-linking to controlled pore-glass using glutaraldehyde. The performance of enzyme reactors packed with the immobilized XO were considered theoretically and investigated experimentally. Using a post-column reactor packed with the immobilized XO, simultaneous determinations of hypoxanthine and xanthine by reversed-phase high-performance liquid chromatography were achieved.