Wastney M E, Wolff J E, Bickerstaffe R, Ramberg C F, Berman M
Aust J Biol Sci. 1983;36(5-6):463-74. doi: 10.1071/bi9830463.
The kinetics of glucose cycling in 24 ewes bearing twins were studied 1 month before term by bolus injections of [6-3H]- and [U-14C]glucose. The function representing glucose carbon recycling was determined by deconvolution of the [3H]glucose from the [14C]glucose decay curves in plasma by using the SAAM and CONSAM programs, and a model for kinetics of glucose cycling was developed. The [3H]glucose data were fitted by four compartments, and an additional three compartments were required to explain recycling. The results show that labelled carbon was still recycling to plasma 2 days after the injection of tracer. By contrast, a similar analysis on a non-pregnant sheep, with data taken from the literature, showed that no more material was recycled after 1 day. It appears that a larger fraction (20 v. 5%) of the carbon 6 of glucose recycles in pregnant than in non-pregnant sheep. This presumably reflects the metabolism by the feto-placental unit and the increased rate of glucose metabolism during pregnancy.
在预产期前1个月，通过静脉注射[6-³H]-和[U-¹⁴C]葡萄糖，对24只怀有双胞胎的母羊体内葡萄糖循环的动力学进行了研究。利用SAAM和CONSAM程序，通过对血浆中[¹⁴C]葡萄糖衰变曲线中的[³H]葡萄糖进行反卷积，确定了代表葡萄糖碳循环的函数，并建立了葡萄糖循环动力学模型。[³H]葡萄糖数据由四个区室拟合，还需要另外三个区室来解释循环情况。结果表明，注射示踪剂2天后，标记碳仍在向血浆中循环。相比之下，对一只非妊娠绵羊进行的类似分析（数据取自文献）表明，1天后就不再有物质循环。看来，与非妊娠绵羊相比，妊娠母羊体内葡萄糖的C6碳循环比例更大（20%对5%）。这大概反映了胎儿-胎盘单位的代谢以及孕期葡萄糖代谢率的增加。
