叶绿体类囊体膜中518纳米处的光依赖性吸收和选择性散射变化。
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes.
作者信息
Thorne S W, Horvath G, Kahn A, Boardman N K
出版信息
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3858-62. doi: 10.1073/pnas.72.10.3858.
Abstract
The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates with the light-induced change in 90 degrees light scattering at 518 nm. Both show a similar time course, similar pH dependence with a maximum at pH 6.0, and similar sensitivity to inhibitors and to treatment of the chloroplasts with a low concentration of glutaraldehyde. Their light minus dark difference spectra are similar with maxima at about 520 nm. It is concluded that they are manifestations of the same phenomenon, and the slow absorbance increase at 518 nm is due to enhanced scattering. It is proposed that the light-induced changes in scattering at 518 nm reflect alterations in selective dispersion, due to proton uptake and conformational changes in the chloroplast thylakoid membrane.
摘要
分离的叶绿体在518nm处光诱导的吸光度变化包括一个快速相和一个在约20秒内完成的缓慢相。518nm吸光度变化的慢成分与518nm处90度光散射的光诱导变化相关。两者都显示出相似的时间进程、相似的pH依赖性（在pH 6.0时最大）以及对抑制剂和用低浓度戊二醛处理叶绿体的相似敏感性。它们的光减去暗差光谱相似，最大值在约520nm处。得出的结论是，它们是同一现象的表现，并且在518nm处吸光度的缓慢增加是由于散射增强。有人提出，518nm处光诱导的散射变化反映了由于质子摄取和叶绿体类囊体膜构象变化导致的选择性分散的改变。
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