从豌豆中快速分离叶绿体后可检测到体内光诱导的叶绿体收缩。
Light-Induced Chloroplast Shrinkage in vivo Detectable After Rapid Isolation of Chloroplasts From Pisum sativum.
作者信息
Nobel P S
机构信息
Department of Botanical Sciences and Molecular Biology Institute, University of California, Los Angeles, California 90024.
出版信息
Plant Physiol. 1968 May;43(5):781-7. doi: 10.1104/pp.43.5.781.
A light-induced shrinkage of chloroplasts in vivo could be detected with chloroplasts isolated within 2 minutes of harvesting pea plants. As determined both by packed volume and Coulter counter, the mean volume of chloroplasts from plants in the dark was 39 mu(3), whereas it was 31 mu(3) for chloroplasts from plants in the light. Upon illumination of the plants, the half-time for the chloroplast shrinkage in vivo was about 3 minutes, and the half-time for the reversal in the dark was about 5 minutes. A plant growth temperature of 20 degrees was optimal for the volume change. The chloroplast shrinkage was half-maximal for a light intensity of 400 lux incident on the plants and was light-saturated near 2000 lux. The light-absorbing pigment responsible for the volume change was chlorophyll. This light-induced shrinkage resulted in a flattening and slight indenting of the chloroplasts. This chloroplast flattening upon illumination of the plants may accompany an increase in the photosynthetic efficiency of chloroplasts.
在收获豌豆植株后2分钟内分离出的叶绿体，可检测到其在体内因光照引起的收缩。通过堆积体积和库尔特计数器测定，黑暗中生长的植株的叶绿体平均体积为39立方微米，而光照下生长的植株的叶绿体平均体积为31立方微米。对植株进行光照时，叶绿体在体内收缩的半衰期约为3分钟，在黑暗中恢复的半衰期约为5分钟。植株生长温度为20摄氏度时，体积变化最为适宜。照射到植株上的光强为400勒克斯时，叶绿体收缩达到最大值的一半，光强接近2000勒克斯时达到光饱和。引起体积变化的吸光色素是叶绿素。这种光照引起的收缩导致叶绿体变扁平并略有凹陷。植株光照后叶绿体的这种变扁平可能伴随着叶绿体光合效率的提高。
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