Naess A, Solberg C O
J Immunol Methods. 1984 Feb 10;66(2):271-6. doi: 10.1016/0022-1759(84)90338-7.
Granulocytes were more sensitive to age variation of EAC3b indicator cells than lymphocytes, and showed a significant decrease in rosette formation with EAC3b indicator cells more than 2 weeks old; whereas lymphocytes showed unchanged EAC3b rosette formation with indicator cells up to 4 weeks old. EAIgG rosette formation by granulocytes was less affected by the age of the indicator cells than by their degree of sensitization, with a 25% loss of rosettes after 1 dilution of the sensitizing antibody. Thus optimal sensitization is essential for granulocyte EAIgG rosette formation, and relatively fresh indicator cells for EAC3b rosette formation. Hemolysis of contaminating erythrocytes did not influence EAIgG or EAC3b rosette formation.
粒细胞对EAC3b指示细胞的年龄变化比淋巴细胞更敏感，并且与超过2周龄的EAC3b指示细胞形成的玫瑰花结明显减少；而淋巴细胞与长达4周龄的指示细胞形成的EAC3b玫瑰花结没有变化。粒细胞形成EAIgG玫瑰花结受指示细胞年龄的影响小于受其致敏程度的影响，致敏抗体稀释1倍后玫瑰花结损失25%。因此，最佳致敏对于粒细胞EAIgG玫瑰花结的形成至关重要，而对于EAC3b玫瑰花结的形成则需要相对新鲜的指示细胞。污染红细胞的溶血不影响EAIgG或EAC3b玫瑰花结的形成。
