Averkina R F
Biull Eksp Biol Med. 1984 Jan;97(1):68-71.
Using specific antirenal sera obtained from rabbits and absorbed with a mixture of extracts from heterologous organs, a specific antigen was detected in human and CBA mouse renal extracts. Its molecular weight was found to amount to about 100 000 dalton. It is salted out with ammonium sulfate at 50-70% saturation of renal extract and is destroyed on extract heating for 30 min at 75 degrees C. This antigen is sensitive to trypsin and papain but resistant to hyaluronidase. It is partially destroyed by DNase and RNase, provided the latter ones are used in comparatively high doses (1 mg per 0.3 ml extract) and exposure lasts one day. Based on the study of the physicochemical properties it is suggested that the kidney-specific antigen may be a ribonucleoprotein or a deoxyribonucleoprotein but cannot be attributed to glycoproteins.
利用从兔子获得并经异源器官提取物混合物吸收的特异性抗肾血清，在人和CBA小鼠的肾提取物中检测到一种特异性抗原。发现其分子量约为100 000道尔顿。它在肾提取物50 - 70%饱和度的硫酸铵中盐析出来，并在75摄氏度下加热提取物30分钟后被破坏。这种抗原对胰蛋白酶和木瓜蛋白酶敏感，但对透明质酸酶有抗性。如果以相对高的剂量（每0.3毫升提取物1毫克）使用并作用一天，它会被DNA酶和RNA酶部分破坏。基于对其理化性质的研究，提示肾脏特异性抗原可能是一种核糖核蛋白或脱氧核糖核蛋白，但不属于糖蛋白。
