Karmali R A, Wustrow T, Thaler H T, Good R A
Cancer Res. 1984 Feb;44(2):467-71.
AKR mice develop spontaneous lymphoid leukemia late (8 to 12 months) in life, although persistent murine leukemia virus production occurs throughout their life. This suggests that age-related changes are involved in development of leukemia. Prostaglandin biosynthesis was therefore studied in 24-hr cultures in vitro at 37 degrees of peritoneal macrophages, splenocytes, thymocytes, bone marrow, and lymph node cells. AKR mice of 2, 6, and 8 to 12 months of age were studied. Prostaglandin E1, prostaglandin E2, prostaglandin F2 alpha, 6-ketoprostaglandin F1 alpha, and thromboxane B2 were measured. In cultures of peritoneal macrophages and cells from spleen, thymus, and lymph nodes, the biosynthesis of all five prostaglandin moieties was higher in those cultures prepared from 8- to 12-month-old spontaneously leukemic mice in comparison with those from 2-month-old nonleukemic AKR mice. However, when leukemia was transplanted in 3-month-old AKR mice, synthesis of all five compounds was reduced significantly in cultures of peritoneal macrophages and splenocytes prepared from these 3-month-old leukemic mice. The present data demonstrate abnormalities in prostaglandin synthesis by various cells of the immune system in leukemic mice. However, the nature of these changes was different in cultures of cells from spontaneously leukemic mice from those with transplanted leukemia. Age-related increases in prostaglandin synthesis by various lymphoid cells from spontaneously leukemic AKR mice (8 to 12 months old) occurred at a much earlier age than in BALB/c mice and may be related to the leukemic condition.
AKR小鼠在生命后期（8至12个月）会自发发生淋巴细胞白血病，尽管其一生中都会持续产生鼠白血病病毒。这表明与年龄相关的变化参与了白血病的发生。因此，在37℃下对腹膜巨噬细胞、脾细胞、胸腺细胞、骨髓和淋巴结细胞进行了24小时体外培养，研究前列腺素的生物合成。对2、6以及8至12月龄的AKR小鼠进行了研究。测定了前列腺素E1、前列腺素E2、前列腺素F2α、6-酮前列腺素F1α和血栓素B2。在腹膜巨噬细胞以及来自脾脏、胸腺和淋巴结的细胞培养物中，与2月龄非白血病AKR小鼠相比，来自8至12月龄自发白血病小鼠的培养物中所有五种前列腺素部分的生物合成更高。然而，当将白血病移植到3月龄AKR小鼠中时，这些3月龄白血病小鼠的腹膜巨噬细胞和脾细胞培养物中所有五种化合物的合成均显著降低。目前的数据表明白血病小鼠免疫系统的各种细胞中前列腺素合成存在异常。然而，自发白血病小鼠细胞培养物中的这些变化性质与移植性白血病小鼠细胞培养物中的不同。自发白血病AKR小鼠（8至12月龄）各种淋巴细胞中前列腺素合成与年龄相关的增加发生的年龄比BALB/c小鼠早得多，并且可能与白血病状况有关。
