Bullick G R, Frizzell R A, Castro G A
Exp Parasitol. 1984 Feb;57(1):104-9. doi: 10.1016/0014-4894(84)90069-9.
Epithelium of isolated small intestinal segments were studied in Ussing-type chambers to detect physiological changes associated with rapid, immune rejection of Trichinella spiralis infective larvae. Electrophysiological parameters associated with Na+-coupled hexose transport were measured. Changes in transepithelial electrical potential difference (PD), resistance, and short circuit current (Isc) due to the addition of actively absorbed beta-methyl-D-glucoside (BMG) to the mucosal solution were determined. Measurements were made prior to and 30 min after primary and secondary infections. Animals were infected by intraduodenal inoculation. As the infective larval dose in primarily infected (nonimmunized) rats increased from 50 to 2000 larvae the magnitude of the rise in Isc elicited by BMG decreased in a dose-dependent fashion, with 50 larvae per rat having no effect. In previously infected (immunized) rats challenged with a secondary inoculum, all doses, ranging from 50 to 2000 larvae per rat, decreased the BMG-stimulated change in Isc by approximately 50%. The effect of 50 worms per rat in immunized hosts was equivalent to that produced by approximately 1600 worms in nonimmunized animals. Measurements of 14C-BMG mucosa-to-serosa flux confirmed that Na+-BMG cotransport was responsible for observed changes in Isc. Results support the conclusion that changes in intestinal epithelial function are associated with larval challenge of immune rats.
在Ussing型小室中研究分离的小肠段上皮，以检测与旋毛虫感染性幼虫快速免疫排斥相关的生理变化。测量与钠耦联己糖转运相关的电生理参数。测定了向黏膜溶液中添加主动吸收的β-甲基-D-葡萄糖苷（BMG）后跨上皮电位差（PD）、电阻和短路电流（Isc）的变化。在初次感染和二次感染之前及之后30分钟进行测量。通过十二指肠内接种使动物感染。随着初次感染（未免疫）大鼠中感染性幼虫剂量从50只增加到2000只，BMG引起的Isc升高幅度呈剂量依赖性降低，每只大鼠接种50只幼虫无影响。在先前感染（免疫）的大鼠中接种二次接种物，所有剂量（每只大鼠50至2000只幼虫）使BMG刺激的Isc变化降低约50%。每只大鼠接种50条虫对免疫宿主的影响相当于未免疫动物接种约1600条虫所产生的影响。14C-BMG从黏膜到浆膜通量的测量证实，钠-BMG共转运是观察到的Isc变化的原因。结果支持以下结论：肠道上皮功能的变化与免疫大鼠的幼虫攻击有关。
