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瘰疬分枝杆菌中的质粒编码汞还原酶。

Plasmid-encoded mercuric reductase in Mycobacterium scrofulaceum.

作者信息

Meissner P S, Falkinham J O

出版信息

J Bacteriol. 1984 Feb;157(2):669-72. doi: 10.1128/jb.157.2.669-672.1984.

DOI:10.1128/jb.157.2.669-672.1984
PMID:6693354
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215301/
Abstract

A Chesapeake Bay water isolate of Mycobacterium scrofulaceum containing a 115-megadalton plasmid (pVT1) grew in the presence of 100 microM HgCl2 and converted soluble 203Hg2+ to volatile mercury at a rate of 50 pmol/10(8) cells per min. Cell extracts contained a soluble mercuric reductase whose activity was not dependent on exogenously supplied thiol compounds. The enzyme displayed nearly identical activity when either NADH or NADPH served as the electron donor. A spontaneously cured derivative lacking pVT1 failed to grow in the presence of 100 microM HgCl2 and possessed no detectable mercuric reductase activity.

摘要

一株含有115兆道尔顿质粒（pVT1）的来自切萨皮克湾水域的瘰疬分枝杆菌在100微摩尔/升氯化汞存在的情况下生长，并以每分钟50皮摩尔/10⁸个细胞的速率将可溶性的203Hg²⁺转化为挥发性汞。细胞提取物含有一种可溶性汞还原酶，其活性不依赖于外源提供的硫醇化合物。当以NADH或NADPH作为电子供体时，该酶表现出几乎相同的活性。一个自发治愈的缺乏pVT1的衍生物在100微摩尔/升氯化汞存在的情况下无法生长，并且没有可检测到的汞还原酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c0b/215301/07129a93eef8/jbacter00237-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c0b/215301/07129a93eef8/jbacter00237-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c0b/215301/07129a93eef8/jbacter00237-0334-a.jpg

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本文引用的文献

1
From the Center for Disease Control. Isolation of nontuberculous mycobacteria in the United States, 1979.来自疾病控制中心。1979年美国非结核分枝杆菌的分离情况。
J Infect Dis. 1980 Nov;142(5):779-83. doi: 10.1093/infdis/142.5.779.
2
Epidemiology of infection by nontuberculous mycobacteria. I. Geographic distribution in the eastern United States.非结核分枝杆菌感染的流行病学。I. 美国东部的地理分布
Am Rev Respir Dis. 1980 Jun;121(6):931-7. doi: 10.1164/arrd.1980.121.6.931.
3
Epidemiology of infection by nontuberculous mycobacteria. II. Growth and survival in natural waters.
在流行的脓肿分枝杆菌亚种 bolletii 菌株中检测和测序一种广泛宿主范围的接合型 IncP-1β 质粒。
PLoS One. 2013;8(4):e60746. doi: 10.1371/journal.pone.0060746. Epub 2013 Apr 2.
4
Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.开发新一代载体，用于分枝杆菌中的基因表达、基因替换和蛋白质-蛋白质相互作用研究。
Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 10.1128/AEM.03695-12. Epub 2013 Jan 11.
5
Sequence and analysis of a plasmid-encoded mercury resistance operon from Mycobacterium marinum identifies MerH, a new mercuric ion transporter.来自海分枝杆菌的质粒编码汞抗性操纵子的序列分析鉴定出一种新的汞离子转运蛋白MerH。
J Bacteriol. 2009 Jan;191(1):439-44. doi: 10.1128/JB.01063-08. Epub 2008 Oct 17.
6
Environmental factors affecting the occurrence of mycobacteria in brook waters.影响泉水中分枝杆菌发生的环境因素。
Appl Environ Microbiol. 1993 Feb;59(2):398-404. doi: 10.1128/aem.59.2.398-404.1993.
7
Decolorization of malachite green and crystal violet by waterborne pathogenic mycobacteria.水生致病性分枝杆菌对孔雀石绿和结晶紫的脱色作用
Antimicrob Agents Chemother. 2003 Jul;47(7):2323-6. doi: 10.1128/AAC.47.7.2323-2326.2003.
8
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Appl Environ Microbiol. 1998 Sep;64(9):3383-8. doi: 10.1128/AEM.64.9.3383-3388.1998.
9
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J Bacteriol. 1997 Apr;179(8):2753-6. doi: 10.1128/jb.179.8.2753-2756.1997.
10
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Clin Microbiol Rev. 1996 Apr;9(2):177-215. doi: 10.1128/CMR.9.2.177.
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Am Rev Respir Dis. 1980 Jul;122(1):89-94. doi: 10.1164/arrd.1980.122.1.89.
4
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J Gen Microbiol. 1981 Dec;127(2):333-8. doi: 10.1099/00221287-127-2-333.
5
Characterization of plasmids from strains of Mycobacterium avium-intracellulare.鸟分枝杆菌-胞内分枝杆菌菌株质粒的特征分析
Rev Infect Dis. 1981 Sep-Oct;3(5):949-52. doi: 10.1093/clinids/3.5.949.
6
Plasmid deoxyribonucleic acid and translucent-to-opaque variation in Mycobacterium intracellulare 103.胞内分枝杆菌103中的质粒脱氧核糖核酸与半透明至不透明变异
J Bacteriol. 1981 May;146(2):656-9. doi: 10.1128/jb.146.2.656-659.1981.
7
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J Bacteriol. 1981 Mar;145(3):1365-73. doi: 10.1128/jb.145.3.1365-1373.1981.
8
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J Infect Dis. 1982 Dec;146(6):829-33. doi: 10.1093/infdis/146.6.829.
9
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Antimicrob Agents Chemother. 1981 Nov;20(5):666-77. doi: 10.1128/AAC.20.5.666.
10
Mechanism of antibiotic resistance in Mycobacterium intracellulare.胞内分枝杆菌的抗生素耐药机制。
Microbiol Immunol. 1983;27(5):425-31. doi: 10.1111/j.1348-0421.1983.tb00601.x.