Ravel J, Schrempf H, Hill R T
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA.
Appl Environ Microbiol. 1998 Sep;64(9):3383-8. doi: 10.1128/AEM.64.9.3383-3388.1998.
The Streptomyces strains CHR3 and CHR28, isolated from the Baltimore Inner Harbor, contained two and one, respectively, giant linear plasmids which carry terminally bound proteins. The plasmids pRJ3L (322 kb), from CHR3, and pRJ28 (330 kb), from CHR28, carry genes homologous to the previously characterized chromosomal Streptomyces lividans 66 operon encoding resistance against mercuric compounds. Both plasmids are transmissible (without any detectable rearrangement) to the chloramphenicol-resistant S. lividans TK24 strain lacking plasmids and carrying a chromosomal deletion of the mer operon. S. lividans TK24 conjugants harboring pRJ3L or pRJ28 exhibited profiles of mercury resistance to mercuric compounds similar to those of Streptomyces strains CHR3 and CHR28.
从巴尔的摩内港分离出的链霉菌菌株CHR3和CHR28分别含有两个和一个携带末端结合蛋白的巨大线性质粒。来自CHR3的质粒pRJ3L（322 kb）和来自CHR28的质粒pRJ28（330 kb）携带与先前鉴定的编码对汞化合物抗性的淡紫链霉菌66染色体操纵子同源的基因。这两种质粒都可以（无任何可检测到的重排）转移到缺乏质粒且mer操纵子染色体缺失的耐氯霉素淡紫链霉菌TK24菌株中。携带pRJ3L或pRJ28的淡紫链霉菌TK24接合体对汞化合物的抗汞谱与链霉菌菌株CHR3和CHR28相似。
