Grosso D S, Larsen B R
J Clin Endocrinol Metab. 1984 Feb;58(2):368-73. doi: 10.1210/jcem-58-2-368.
Estrogen metabolism was studied in a newly established cell line (RL95-2) derived from a human endometrial carcinoma. Estradiol and estrone were metabolized to water-soluble derivatives by cells under in vitro culture conditions. Between 80-90% of the added steroids were metabolized, with nearly quantitative recovery of the products from the incubation medium. Arylsulfatase treatment converted the metabolites to ether-soluble forms, whereas beta-glucuronidase had no effect on the aqueous solubility of these compounds. Butanol extracts of the water-soluble estradiol metabolites cochromatographed on high performance liquid chromatography with 17 beta-estradiol-3-sulfate (93.6%) or estrone-3-sulfate (3.5%). No more than 6% of the estradiol added to the incubation medium was recovered in the form of estrone, either as estrone or estrone sulfate. After arylsulfatase treatment of the estradiol conjugates, 92% of the ether-soluble radioactivity cochromatographed with estradiol, and 3.8% cochromatographed with estrone. Estrogen-sulfurylating activity was localized in the cytosol of subcellular fractions of RL95-2 cells. The sulfoconjugation of estrogens by RL95-2 cells may prove useful as a model for the investigation of estrogen metabolism in endometrial carcinoma cells.
在一种新建立的源自人子宫内膜癌的细胞系（RL95 - 2）中研究了雌激素代谢。在体外培养条件下，细胞将雌二醇和雌酮代谢为水溶性衍生物。添加的类固醇中有80 - 90%被代谢，培养介质中产物的回收率几乎是定量的。芳基硫酸酯酶处理将代谢物转化为醚溶性形式，而β - 葡萄糖醛酸酶对这些化合物的水溶性没有影响。水溶性雌二醇代谢物的丁醇提取物在高效液相色谱上与17β - 雌二醇 - 3 - 硫酸盐（93.6%）或雌酮 - 3 - 硫酸盐（3.5%）共色谱。添加到培养介质中的雌二醇以雌酮或硫酸雌酮形式回收的量不超过6%。对雌二醇缀合物进行芳基硫酸酯酶处理后，92%的醚溶性放射性与雌二醇共色谱，3.8%与雌酮共色谱。雌激素硫酸化活性定位于RL95 - 2细胞亚细胞组分的胞质溶胶中。RL95 - 2细胞对雌激素的硫酸化结合可能被证明是研究子宫内膜癌细胞中雌激素代谢的有用模型。
