用鲎试剂评估去除干扰素和淋巴因子制剂中污染内毒素的吸收情况。

Evaluation of absorption with Limulus amebocyte lysate to remove contaminating endotoxin from interferon and lymphokine preparations.

作者信息

Biondi A, Landolfo S, Fumarola D, Polentarutti N, Introna M, Mantovani A

出版信息

J Immunol Methods. 1984 Jan 20;66(1):103-12. doi: 10.1016/0022-1759(84)90253-9.

Abstract

Alpha and beta human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

摘要

α和β人干扰素（IFN）制剂以及淋巴因子（PHA刺激的血液淋巴细胞的上清液）被故意用内毒素（20 ng/ml）污染，随后通过用鲎试剂（LAL）吸附使其无内毒素。在8次实验中，用LAL吸附并未明显影响IFN和淋巴因子的抗病毒活性，在两次实验中导致了30% - 50%的降低。这些制剂刺激自然杀伤细胞活性和单核细胞细胞毒性的能力并未因在LAL上的吸附而持续改变。当测量淋巴因子对单核细胞的趋化活性时，诱导迁移的单核细胞最大数量和最大活性淋巴因子浓度不受LAL吸附的影响。然而，经LAL处理的淋巴因子显示出前带现象，推测与从LAL凝胶中释放趋化抑制因子有关。