Fennell C L, Totten P A, Quinn T C, Patton D L, Holmes K K, Stamm W E
J Infect Dis. 1984 Jan;149(1):58-66. doi: 10.1093/infdis/149.1.58.
Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-micrograms disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.
对从同性恋男性直肠培养物中分离出的13株弯曲杆菌样微生物（CLOs）进行了研究。与过氧化氢酶阳性弯曲杆菌属菌种一样，CLOs是弯曲的革兰氏阴性杆菌，不能在有氧条件下生长，具有运动性，氧化酶和过氧化氢酶呈阳性，且不利用葡萄糖。然而，CLOs无法归入任何弯曲杆菌属菌种，因为它们生长缓慢且具有异常的菌落形态；在25℃下不生长，不水解马尿酸盐，在三糖铁琼脂中不产生H2S，也不耐受2%氯化钠；对30微克的萘啶酸纸片敏感；耐受1%甘氨酸和0.04%氯化三苯基四氮唑。根据硝酸盐还原、42℃生长情况以及对头孢噻吩的敏感性差异，鉴定出三组CLOs。通过菌落杂交技术，从每个CLO组的一株菌株中分离出的全细胞DNA与同一组中其他菌株的DNA杂交，但不与其他组的菌株或过氧化氢酶阳性弯曲杆菌属菌种的参考菌株杂交。
