Vitamin E concentrations in the brains and some selected peripheral tissues of selenium-deficient and vitamin E-deficient mice.

Weanling male CD-l mice were fed control, vitamin E-deficient or selenium-deficient diets for periods of 12 to 20 weeks. alpha-Tocopherol concentrations in plasma, liver, and testes, as well as in three specific areas in the brain (cerebral hemisphere, cerebellum, and medulla plus pons) were determined by high performance liquid chromatography. Significant concentrations of alpha-tocopherol were found in all brain samples from vitamin E-deficient animals long after the peripheral tissues were depleted, indicating that brain is more resistant to vitamin E deficiency than peripheral tissues. Cerebellar concentrations of alpha-tocopherol were consistently lower than those of cerebral hemisphere and medulla-pons. Furthermore, the cerebellar alpha-tocopherol concentration sustained a larger decline than the other two brain areas within 6 weeks of vitamin E deficiency treatment. These and other data suggest that cerebellum may be more susceptible to damage from vitamin E deficiency than other parts of the brain. Selenium deficiency did not affect brain alpha-tocopherol concentrations during the 12 weeks of the study.