South P K, Smith A D, Guidry C A, Levander O A
United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Nutrient Requirements and Functions Laboratory, Beltsville, MD 20705, USA.
Biol Trace Elem Res. 2006 Mar;109(3):293-300. doi: 10.1385/BTER:109:3:293.
Physical restraint has been associated with increased oxidative damage to lipid, protein, and DNA. The purpose of this experiment was to determine whether physical restraint would further exacerbate oxidative stress in mice fed a selenium (Se) and vitamin E (VE) deficient diet. Three-week- old mice were fed a Torula yeast diet containing adequate or deficient Se and VE. Menhaden oil was added to the deficient diet to impose an additional oxidative stress. After 4 wk feeding, half the mice in each group were restrained for 5 d in well-ventilated conical tubes for 8 h daily. Mice fed the Se and VE deficient diets had increased liver thiobarbituric acid-reactive substance (TBARS) levels and decreased liver glutathione peroxidase (GPX1) activity and alpha-tocopherol levels. Plasma corticosterone levels were elevated in restrained mice fed the deficient diet compared to unrestrained mice fed the adequate diet. Restraint had no effect on liver TBARS or alpha-tocopherol levels. Liver GPX1 activity, however, was lower in restrained mice fed the adequate diet. In addition, liver superoxide dismutase (SOD) activity was lower in the restrained mice fed the adequate or deficient diet. Thus, under our conditions, Se and VE deficient diet, but not restraint, increased lipid peroxidation in mice. Restraint, however, decreased antioxidant protection in mice due to decreased activities of GPX1 and SOD enzymes.
身体束缚已被证实与脂质、蛋白质和DNA的氧化损伤增加有关。本实验的目的是确定身体束缚是否会进一步加剧喂食缺硒（Se）和维生素E（VE）饮食的小鼠的氧化应激。给三周龄的小鼠喂食含有充足或缺乏Se和VE的圆酵母饮食。在缺乏饮食中添加鲱鱼油以施加额外的氧化应激。喂食4周后，每组中的一半小鼠每天在通风良好的锥形管中束缚5天，每天8小时。喂食Se和VE缺乏饮食的小鼠肝脏硫代巴比妥酸反应性物质（TBARS）水平升高，肝脏谷胱甘肽过氧化物酶（GPX1）活性和α-生育酚水平降低。与喂食充足饮食的未束缚小鼠相比，喂食缺乏饮食的束缚小鼠血浆皮质酮水平升高。束缚对肝脏TBARS或α-生育酚水平没有影响。然而，喂食充足饮食的束缚小鼠肝脏GPX1活性较低。此外，喂食充足或缺乏饮食的束缚小鼠肝脏超氧化物歧化酶（SOD）活性较低。因此，在我们的条件下，缺硒和维生素E的饮食而非束缚会增加小鼠的脂质过氧化。然而，由于GPX1和SOD酶活性降低，束缚会降低小鼠的抗氧化保护作用。
