Sprinkle D J, Hassan A S, Subbiah M T
Proc Soc Exp Biol Med. 1984 Mar;175(3):386-97. doi: 10.3181/00379727-175-41811.
Chenodeoxycholic acid (CDCA) was fed to pregnant rats at the 0.25% level in the diet from Day 11 of gestation to delivery in order to evaluate the effects on (1) maternal tissue bile acid composition, (2) neonatal tissue bile acid composition and cholesterol-7 alpha-hydroxylase activity, and (3) maternal, neonatal, and postnatal liver morphology. Feeding CDCA increased maternal lithocholic acid while significantly decreasing deoxycholic acid, cholic acid, and total bile acids. Feeding CDCA resulted in a significantly higher chenodeoxycholic acid pool in the neonates while neonatal plasma cholesterol and the 7 alpha-hydroxylation of cholesterol was not significantly affected. Morphological examination of maternal, neonatal, and postnatal rat liver revealed no significant hepatotoxicity. This investigation has shown that (a) neonates of CDCA fed dams have a significantly greater pool of CDCA, suggesting maternal-to-fetal transfer of dihydroxy bile acids, (b) neonatal cholesterol-7 alpha-hydroxylase activity and total tissue bile acid pools are not significantly altered by increased pool of CDCA, and (c) no hepatotoxic effects on maternal, neonatal, and postnatal livers were evident with gestational feeding of CDCA at the 0.25% level in the rat.
从妊娠第11天到分娩，以0.25%的水平将鹅去氧胆酸（CDCA）添加到妊娠大鼠的饮食中，以评估其对以下方面的影响：（1）母体组织胆汁酸组成；（2）新生组织胆汁酸组成和胆固醇7α-羟化酶活性；（3）母体、新生和产后肝脏形态。喂食CDCA会增加母体石胆酸，同时显著降低脱氧胆酸、胆酸和总胆汁酸。喂食CDCA会使新生儿的鹅去氧胆酸池显著增加，而新生儿血浆胆固醇和胆固醇的7α-羟化未受到显著影响。对母体、新生和产后大鼠肝脏的形态学检查未发现明显的肝毒性。本研究表明：（a）喂食CDCA的母鼠所产新生儿的CDCA池显著更大，提示二羟基胆汁酸存在母胎转移；（b）CDCA池增加并未显著改变新生儿胆固醇7α-羟化酶活性和总组织胆汁酸池；（c）在大鼠中，以0.25%的水平在妊娠期喂食CDCA对母体、新生和产后肝脏没有明显的肝毒性作用。
