Hoffmann G E, Weiss L
Hoppe Seylers Z Physiol Chem. 1978 Jun;359(6):733-40. doi: 10.1515/bchm.1978.359.1.733.
Methods are described for the direct optical assay of citrate, acetate, and acetoacetate production by isolated, incubated rat liver mitochondria. Each metabolite is converted into acetyl-CoA, using ATP: citrate lyase or acetyl-CoA synthetase or acetoacetyl-CoA synthetase and acetyl-CoA acetyltransferase, respectively. Arylamine acetyltransferase acts as auxiliary enzyme. It was shown that isolated rat liver mitochondria produce citrate, acetate and acetoacetate, and that production rates are stimulated by pyruvate and hexanoate. It was concluded that these three products might contribute to the transport of acetyl units across the mitochondrial membrane and thus serve as precursors in fatty acid synthesis. The rate of acetyl transfer does not seem to be rate-limiting with regard to the overall-process of fatty acid synthesis from carbohydrates.
本文描述了通过分离并孵育大鼠肝脏线粒体来直接光学测定柠檬酸盐、乙酸盐和乙酰乙酸盐生成的方法。每种代谢物分别使用ATP：柠檬酸裂解酶或乙酰辅酶A合成酶或乙酰乙酰辅酶A合成酶和乙酰辅酶A乙酰转移酶转化为乙酰辅酶A。芳胺乙酰转移酶作为辅助酶。结果表明，分离的大鼠肝脏线粒体可产生柠檬酸盐、乙酸盐和乙酰乙酸盐，且丙酮酸和己酸盐可刺激其生成速率。得出的结论是，这三种产物可能有助于乙酰单位穿过线粒体膜的转运，从而作为脂肪酸合成的前体。就从碳水化合物合成脂肪酸的整个过程而言，乙酰转移速率似乎不是限速因素。
