Vijay H M, Lavergne G, Ashton F E, Ryan A, Diena B B
Br J Vener Dis. 1977 Apr;53(2):106-8. doi: 10.1136/sti.53.2.106.
Outbred Wistar rats were immunised with a single intraperitoneal injection of a mixture of 30 mg of A1(OH)3 and 100microng of gonococcal zeolite antigen (ZA). Ten days after immunisation, ZA prepared from T1 and T4 colonies of Neisseria gonorrhoeae strain GC6 (GC6-T1 ZA and GC6-T4 ZA) were able to elicit a reaginic (IgE) response which declined to low levels by 35 days. There was a significant amount of histamine release from the mast cells of actively-sensitised rats on challenge with a specific gonococcal antigen. The antisera heated at a temperature of 56 degrees C for four hours failed to elicit a passive cutaneous anaphylaxis (PCA) reaction, indicating a lack of IgGa antibody. In addition antisera to GC6-T1 ZA gave a positive PCA reaction with GC6-T4 ZA and ZA prepared from T1 colonies of six heterologous gonococcal strains suggesting that these strains of N. gonorrhoeae share common antigenic determinants.
将30毫克氢氧化铝和100微克淋病菌沸石抗原（ZA）的混合物通过腹腔内单次注射对远交系Wistar大鼠进行免疫。免疫后10天，由淋病奈瑟菌GC6菌株的T1和T4菌落制备的ZA（GC6-T1 ZA和GC6-T4 ZA）能够引发反应素（IgE）反应，到35天时该反应降至低水平。用特异性淋病菌抗原攻击时，主动致敏大鼠的肥大细胞会释放大量组胺。在56摄氏度温度下加热4小时的抗血清未能引发被动皮肤过敏反应（PCA），表明缺乏IgGa抗体。此外，针对GC6-T1 ZA的抗血清与GC6-T4 ZA以及由六种异源淋病菌株的T1菌落制备的ZA产生了阳性PCA反应，这表明这些淋病奈瑟菌菌株具有共同的抗原决定簇。
