Petkov D D, Stoineva I
Biochem Biophys Res Commun. 1984 Jan 13;118(1):317-23. doi: 10.1016/0006-291x(84)91103-3.
The partitioning of the acylenzyme acetyl-(Gly)n-Phe(NO2)-chymotrypsin (n = 0,1,2) to peptide and peptide acid is observed spectrophotometrically. Values of partitioning ratios for various nucleophiles are calculated from the spectral data. They are a measure for the "true" nucleophile reactivity and are useful in the prediction of the best experimental conditions in enzymic peptide synthesis. A large difference in the nucleophile reactivity is observed which is attributed to the S'2-P'2 interaction.
通过分光光度法观察了酰基酶乙酰基-（甘氨酸）n-苯丙氨酸（硝基）-胰凝乳蛋白酶（n = 0,1,2）向肽和肽酸的分配情况。根据光谱数据计算了各种亲核试剂的分配比数值。它们是“真实”亲核试剂反应性的一种度量，并且在预测酶促肽合成的最佳实验条件方面很有用。观察到亲核试剂反应性存在很大差异，这归因于S'2-P'2相互作用。
