hCG-Increased phosphorylation of proteins in primary culture of Leydig cells: further characterization.

Previous studies using monodimensional gel electrophoresis of total cell proteins have shown that hCG was able to increase the phosphorylation of at least 6 proteins in cultured porcine Leydig cells. By using subcellular fractionation and 2D gel electrophoresis, we now show that most of these proteins, whose phosphorylation was increased by hCG, are basic nuclear proteins. In addition, hCG also increases the phosphorylation of microsomal proteins in particular ribosomal protein S6. Moreover no phosphoproteins were detected in the cytosol.