Shimamura M, Endo T, Inoue Y, Inoue S, Kambara H
Biochemistry. 1984 Jan 17;23(2):317-22. doi: 10.1021/bi00297a022.
A series of acidic oligosaccharide alditols having different neutral core oligosaccharides were isolated from salmon egg polysialoglycoproteins by alkali-borohydride treatment followed by anion-exchange chromatography and Iatrobead chromatography. Their structures were determined by methylation analysis, molecular secondary ion mass spectrometry of underivatized oligosaccharides, and enzymatic desialylation. The molecular secondary ion mass spectra of intact sialooligosaccharides exhibit pronounced quasi-molecular-ion peaks, (M + H)+, (M + Na)+, (M + 2Na - H)+, and/or (M + K)+, as well as some diagnostic sequence ion peaks. Of a number of oligosaccharide alditols, the following are novel: Fuc alpha 1 leads to 3GalNAc beta l1 leads to 3Gal beta 1 leads to 4Gal beta 1 leads to 3[(leads to 8NeuGc alpha 2)n leads to 6]GalNAcol (n = 1-6). The proton nuclear magnetic resonance spectra of these oligosaccharides are also reported and discussed.
通过碱硼氢化物处理，然后进行阴离子交换色谱和离子交换树脂色谱，从鲑鱼卵多唾液酸糖蛋白中分离出一系列具有不同中性核心寡糖的酸性寡糖糖醇。通过甲基化分析、未衍生化寡糖的分子二次离子质谱和酶促去唾液酸化来确定它们的结构。完整唾液酸寡糖的分子二次离子质谱显示出明显的准分子离子峰，(M + H)+、(M + Na)+、(M + 2Na - H)+和/或(M + K)+，以及一些诊断性序列离子峰。在许多寡糖糖醇中，以下是新的：Fucα1→3GalNAcβ1→3Galβ1→4Galβ1→3[(→8NeuGcα2)n→6]GalNAcol（n = 1 - 6）。还报道并讨论了这些寡糖的质子核磁共振谱。
