Costa N D, Stevenson P M
Biochim Biophys Acta. 1984 Feb 9;792(2):130-4. doi: 10.1016/0005-2760(84)90213-3.
Changes in the concentrations of total coenzyme A, acetyl CoA, free carnitine and acetylcarnitine were measured in ovaries from immature rats before and after superovulation with 50 I.U. pregnant mare's serum gonadotropin. In addition, the concentrations of total CoA and total acid-soluble carnitine were measured in liver, adrenal glands and skeletal muscle from the same rats. Ovarian concentrations of total CoA, free carnitine and acetylcarnitine increased 3-fold on gonadotropin stimulation, whereas there was no marked change in total CoA and acid-soluble carnitine concentrations in the other organs. In ovary, the ratio of free CoA to acetyl CoA was about 2:1 during the growth period of follicular development and during active steroidogenesis in the luteal phase, but less than 1 when replication stopped and ovulation occurred. These results show that during periods of high energy demand the ovary has a good capacity to accommodate fatty acid oxidation, and supports the evidence that fatty acids are the major source of reducing equivalents for steroidogenesis at these times.
用50国际单位孕马血清促性腺激素对未成熟大鼠进行超排卵前后，测定其卵巢中总辅酶A、乙酰辅酶A、游离肉碱和乙酰肉碱的浓度变化。此外，还测定了同一批大鼠肝脏、肾上腺和骨骼肌中总辅酶A和总酸溶性肉碱的浓度。促性腺激素刺激后，卵巢中总辅酶A、游离肉碱和乙酰肉碱的浓度增加了3倍，而其他器官中总辅酶A和酸溶性肉碱的浓度没有明显变化。在卵巢中，卵泡发育生长期和黄体期活跃的类固醇生成过程中，游离辅酶A与乙酰辅酶A的比例约为2:1，但在复制停止和排卵发生时，该比例小于1。这些结果表明，在高能量需求时期，卵巢具有良好的脂肪酸氧化能力，并支持脂肪酸是此时类固醇生成中还原当量主要来源的证据。
