Smalheiser N R, Crain S M, Reid L M
Brain Res. 1984 Jan;314(1):136-40. doi: 10.1016/0165-3806(84)90184-6.
Fetal mouse retinal ganglion cell axons have been shown to ramify within co-cultured basement membrane secreting tumor explants and upon isolated basement membranes. Here we report that laminin, a glycoprotein found in basement membranes and adhesion sites of a variety of cell types, acted as a substrate for retinal explant attachment and axonal outgrowth. When axons were given a direct choice, laminin was preferred over collagen. Under suitable conditions (air-dried upon underlying collagen gels), laminin was more effective than fibronectin for promoting axon emergence from retinal explants. These findings have implications for the study of molecular mechanisms underlying CNS axonal outgrowth.
已表明，胎鼠视网膜神经节细胞轴突会在共培养的分泌基底膜的肿瘤外植体以及分离的基底膜内分支。在此，我们报告层粘连蛋白，一种存在于基底膜和多种细胞类型黏附位点的糖蛋白，可作为视网膜外植体附着和轴突生长的底物。当轴突有直接选择时，相较于胶原蛋白，层粘连蛋白更受青睐。在合适条件下（在下面的胶原凝胶上空气干燥），层粘连蛋白在促进轴突从视网膜外植体长出方面比纤连蛋白更有效。这些发现对中枢神经系统轴突生长潜在分子机制的研究具有启示意义。
