Saitô H, Tabeta R, Kodama M, Nagata C, Sato Y
Cancer Lett. 1984 Feb;22(1):65-9. doi: 10.1016/0304-3835(84)90044-2.
In order to elucidate the manner of interaction of 12-O-tetradecanoylphorbol-13-acetate (TPA), 2H-NMR spectra of [20-2H1]TPA and 31P-NMR spectra were recorded in the presence of multibilayers of dimyristoylphosphatidylcholine (DMPC). Observation of several pairs of quadrupole splittings directly proves the TPA molecules are intercalated into the multibilayers. Further, reduction of the 31P chemical shift anisotropy of DMPC multibilayers by TPA is more pronounced than that of phorbol 12,13-diacetate (PDA) whose activity of tumor promotion is very weak.
为了阐明12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）的相互作用方式，在二肉豆蔻酰磷脂酰胆碱（DMPC）多层膜存在的情况下记录了[20 - 2H1]TPA的2H - NMR光谱和31P - NMR光谱。对几对四极分裂的观察直接证明TPA分子插入到多层膜中。此外，TPA对DMPC多层膜的31P化学位移各向异性的降低比肿瘤促进活性非常弱的佛波醇12,13 - 二乙酸酯（PDA）更明显。
