Anteunis A, Pouchelet M, Gansmüller A, Robineaux R
Cell Tissue Res. 1984;235(1):65-70. doi: 10.1007/BF00213724.
The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.
通过使用低pH值的氧化二氨基联苯胺（DAB）作为核酸的差异对比染色剂，并利用连续切片重建技术，研究了豚鼠淋巴细胞在植物血凝素（PHA）刺激过程中核仁DNA空间组织的超微结构变化。位于纤维区内部的延伸DNA细丝起源于与核仁紧密相关的大量异染色质聚集物，以及浓缩染色质的核仁周围壳层。有人认为，染色质的这两个不同区域可能与不同功能相关。
