Bugugnani M J, Koffigan M, Kora I, Ouvry D, Clavey V, Fruchart J C
Clin Chem. 1984 Mar;30(3):349-51.
We describe an isoelectric-focusing method for rapidly separating and quantifying apolipoprotein (apo) C-II and C-III subspecies of triglyceride-rich lipoproteins. Concentrated very-low-density lipoproteins (VLDL) or delipidated apo VLDL are focused on dry acrylamide plates, after their rehydration with ampholytes and urea. Apo C-II and apo C-III in VLDL are resolved into four major bands--C-II (pI 5.01), C-III0 (pI 5.10), C-III1 (pl 4.92), and C-III2 (pI 4.84)--at the same pI values as for purified apo C. This precise technique can be used without delipidating VLDL. The relative percentage of C apoproteins found in VLDL from plasma of normal subjects agreed with previously published data. The ratio of apo C-II to apo C-III decreased in patients with chronic renal failure or with coronary artery disease.
我们描述了一种等电聚焦方法，用于快速分离和定量富含甘油三酯脂蛋白的载脂蛋白（apo）C-II和C-III亚类。浓缩的极低密度脂蛋白（VLDL）或脱脂的apo VLDL在用两性电解质和尿素复水后，在干燥的丙烯酰胺板上进行聚焦。VLDL中的apo C-II和apo C-III可分离为四条主要条带——C-II（pI 5.01）、C-III0（pI 5.10）、C-III1（pl 4.92）和C-III2（pI 4.84）——其pI值与纯化的apo C相同。该精确技术无需对VLDL进行脱脂即可使用。正常受试者血浆中VLDL中C载脂蛋白的相对百分比与先前发表的数据一致。慢性肾功能衰竭或冠状动脉疾病患者的apo C-II与apo C-III的比值降低。
