Wiebe D A, Bernert J T
Clin Chem. 1984 Mar;30(3):352-6.
Esterolytic activity of three enzymic cholesterol reagents was evaluated with use of human serum-based reference materials. Primary cholesterol standards were used to calibrate the enzymic methods, and incomplete cholesteryl ester hydrolysis was measured as the bias between the enzymic cholesterol values and corresponding reference values. The average biases observed with eight reference specimens were 0.15, -1.16, and -7.24%, respectively, for a commercial enzymic reagent with microbial cholesterol esterase, a commercial reagent with an animal source of esterase, and a Centers for Disease Control (CDC) reagent preparation with a microbial cholesterol esterase. By liquid chromatography, we examined the respective specificities of the three esterase reagent preparations and found that the preparations differ in the rate and specificity of catalysis of cholesteryl ester hydrolysis. The CDC reagent was only partly active towards cholesteryl arachidonate, which probably accounts for most of the negative bias of this reagent. Esterolytic activity of the various enzymic reagents is related to the source and matrix of the cholesterol esterase and must be considered with respect to proper selection of calibration materials for serum cholesterol measurement.
使用基于人血清的参考物质评估了三种酶法胆固醇试剂的酯解活性。用一级胆固醇标准品校准酶法，将酶法胆固醇值与相应参考值之间的偏差作为胆固醇酯不完全水解的指标进行测定。对于含有微生物胆固醇酯酶的市售酶试剂、含有动物源酯酶的市售试剂以及含有微生物胆固醇酯酶的疾病控制中心（CDC）试剂制剂，用八个参考标本观察到的平均偏差分别为0.15%、-1.16%和-7.24%。通过液相色谱法，我们研究了三种酯酶试剂制剂各自的特异性，发现这些制剂在胆固醇酯水解催化的速率和特异性方面存在差异。CDC试剂对花生四烯酸胆固醇酯仅有部分活性，这可能是该试剂大部分负偏差的原因。各种酶试剂的酯解活性与胆固醇酯酶的来源和基质有关，在为血清胆固醇测量正确选择校准材料时必须予以考虑。
