Adejuwon C A, Segal S J
Clin Chim Acta. 1984 Mar 13;137(3):245-53. doi: 10.1016/0009-8981(84)90112-8.
Thirty-three of forty-one non-placental human tissue samples analysed by radioimmunoassay (RIA) appeared to contain immunoreactive hCG-like material. Tissues with apparent hCG in appreciable quantity were exposed to heat or protease inhibitors to ascertain the contribution of proteolytic enzyme activity to the observed immunoreactivity. Following incubation with protease inhibitor N-alpha-p-tosyl-L-lysine chloromethyl ketone-HCl (TLCK) at a dose that has no effect on the hCG standard curve and reduces the assayable hCG in term placenta by less than 20%, the hCG-like immunoreactivity of testicular and colon tissues was reduced by 39 to 90%. Similarly, heat treatment virtually eliminated hCG-like immunoreactivity of non-placental normal tissues, but had little effect on purified hCG or hCG present in term placenta. The factor present in normal non-placental human tissue giving positive results in the RIA for hCG is not identical to native hCG; it may be proteolytic in nature thus influencing the radioligand assay. The residual immunoreactivity measured following heat treatment of purified hCG increases when anti hCG-beta antiserum is used, but decreases when either anti hCG-alpha or anti-hCG is used.
通过放射免疫测定法（RIA）分析的41份非胎盘人体组织样本中，有33份似乎含有免疫反应性类人绒毛膜促性腺激素（hCG）物质。将含有大量明显hCG的组织暴露于高温或蛋白酶抑制剂中，以确定蛋白水解酶活性对观察到的免疫反应性的影响。在用对hCG标准曲线无影响且使足月胎盘可检测的hCG降低不到20%的剂量的蛋白酶抑制剂N-α-对甲苯磺酰-L-赖氨酸氯甲基酮盐酸盐（TLCK）孵育后，睾丸和结肠组织的类hCG免疫反应性降低了39%至90%。同样，热处理几乎消除了非胎盘正常组织的类hCG免疫反应性，但对纯化的hCG或足月胎盘中的hCG影响很小。在hCG的RIA中呈阳性结果的正常非胎盘人体组织中存在的因子与天然hCG不同；它可能在性质上是蛋白水解性的，从而影响放射性配体测定。使用抗hCG-β抗血清时，纯化hCG热处理后测得的残留免疫反应性会增加，但使用抗hCG-α或抗hCG时则会降低。
