Ansevin A T, Vizard D L
Comput Biomed Res. 1984 Feb;17(1):15-26. doi: 10.1016/0010-4809(84)90003-x.
Two modes of data processing are appropriate in conducting high resolution thermal denaturation experiments (thermal increments of 0.05 degrees or closer). In the first mode, a general purpose microcomputer provides on-line services important to the control and monitoring of the initial experiment, including control of the spectrophotometer and heater, the recording of data, and the display of current hyperchromicities and approximate first derivatives. A subsequent microcomputer program then reads the recorded data files and carries out accurate calculations of derivative denaturation profiles and the estimate of the statistical error of the first derivative at each point. The data collection program handles three samples at a time and was designed to provide optimal results in thermal denaturation experiments with a single-beam spectrophotometer.
在进行高分辨率热变性实验（热增量为0.05摄氏度或更接近）时，有两种数据处理模式是合适的。在第一种模式中，一台通用微型计算机提供对初始实验的控制和监测至关重要的在线服务，包括分光光度计和加热器的控制、数据记录以及当前增色性和近似一阶导数的显示。随后的一个微型计算机程序接着读取记录的数据文件，并对导数变性图谱进行精确计算以及估计每个点处一阶导数的统计误差。数据收集程序一次处理三个样品，并且设计用于在单光束分光光度计的热变性实验中提供最佳结果。
