Faulstich H, Münter K, Mayer D
FEBS Lett. 1984 Feb 27;167(2):241-4. doi: 10.1016/0014-5793(84)80134-9.
In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol 45Ca2+ per mg protein. In the presence of 1 microM phalloidin, the exchanged amount of 45Ca2+ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the 45Ca2+ deficit caused by phalloidin occurs in the FCCP-sensitive compartment, i.e., the mitochondria.
在3小时的孵育过程中，大鼠肝细胞原代培养物附着于基质上，每毫克蛋白质交换约2.2纳摩尔的45Ca2+。在存在1微摩尔鬼笔环肽的情况下，发现45Ca2+的交换量减少了约30%。使用解偶联剂FCCP和离子载体A23187进行进一步表征，我们确定鬼笔环肽引起的45Ca2+缺乏发生在对FCCP敏感的区室，即线粒体中。
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