用 McCoy 细胞单层和细胞悬液检测艰难梭菌毒素并与 HeLa 细胞检测法进行比较。
Detection of Clostridium difficile toxin with McCoy cell monolayers and cell suspensions and comparison with HeLa cell assay.
作者信息
Maniar A C, Chubb H, Louie T J, Williams T W, Forsyth W, Wilt J C
出版信息
J Clin Microbiol. 1984 Feb;19(2):294-5. doi: 10.1128/jcm.19.2.294-295.1984.
McCoy cell monolayers were compared with HeLa cell monolayers for the detection of Clostridium difficile toxin in 301 stool samples. Tests were positive (greater than or equal to 1/100 dilution) in 83 and 81 specimens tested with McCoy and HeLa cell monolayers, respectively. McCoy cell suspensions were compared with HeLa cell monolayers in 532 stool filtrates. Overall, 90 positive specimens were within one dilution and 432 filtrates were negative with either test, giving a correlation coefficient of r = 0.98. McCoy cell monolayers or suspensions may be a satisfactory substitute for the detection of C. difficile toxin in clinical specimens.
在301份粪便样本中，比较了 McCoy 细胞单层与 HeLa 细胞单层用于检测艰难梭菌毒素的情况。分别用 McCoy 细胞单层和 HeLa 细胞单层检测的83份和81份标本检测呈阳性（稀释度大于或等于1/100）。在532份粪便滤液中比较了 McCoy 细胞悬液与 HeLa 细胞单层。总体而言，90份阳性标本在一个稀释度范围内，432份滤液两种检测均为阴性，相关系数r = 0.98。McCoy 细胞单层或悬液可能是临床标本中检测艰难梭菌毒素的满意替代物。
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