Khawaja J A, Lindholm D B
J Neurochem. 1984 Apr;42(4):930-3. doi: 10.1111/j.1471-4159.1984.tb12693.x.
Rough endoplasmic reticulum (RER) prepared from bulk-isolated neuronal perikarya of rat brain cortex of different postnatal ages was found to be active in vectorial discharge of nascent proteins through the membrane; this activity increased with the increasing age of animals and reached maximal values in adults. RER isolated from whole cortical tissue (containing all cell types) exhibited vectorial release only up to 18 days of age; the preparation from adult animals was essentially devoid of secretory activity. Controlled proteolysis of various preparations suggested that in neuronal RER of 8-day-old rats the proportion of nascent proteins operationally retained in the intravesicular space was about twice that retained by cortical preparations. For the purpose of comparison, these parameters were studied also in liver RER.
从不同出生后年龄的大鼠大脑皮质大量分离的神经元胞体中制备的粗面内质网（RER），被发现其在通过膜向新生蛋白质进行向量性分泌方面具有活性；这种活性随着动物年龄的增加而增强，并在成年动物中达到最大值。从整个皮质组织（包含所有细胞类型）分离的RER仅在18日龄前表现出向量性释放；成年动物的制备物基本上没有分泌活性。对各种制备物进行的可控蛋白水解表明，在8日龄大鼠的神经元RER中，新生蛋白质在囊泡内空间中功能性保留的比例约为皮质制备物保留比例的两倍。为作比较，这些参数也在肝脏RER中进行了研究。
