从纤细短膜虫中分离DNA聚合酶并进行部分特性鉴定。

Isolation and partial characterization of DNA polymerases from Crithidia fasciculata.

作者信息

Holmes A M, Cheriathundam E, Kalinski A, Chang L M

出版信息

Mol Biochem Parasitol. 1984 Feb;10(2):195-205. doi: 10.1016/0166-6851(84)90007-0.

Abstract

Two types of DNA polymerase activity were partially purified from Crithidia fasciculata. The alpha-type, DNA polymerase A, was of high molecular weight and sensitive to N-ethylmaleimide, whereas the beta-type, DNA polymerase B, was of low molecular weight and resistant to N-ethylmaleimide. Phosphocellulose chromatography revealed multiple peaks of DNA polymerase A activity the properties of which, such as pH optimum, salt sensitivity, utilization of synthetic template-initiator complexes and response to DNA polymerase inhibitors were similar. The response of the C. fasciculata DNA polymerase A enzymes to some of these inhibitors and utilization of poly(rA) X oligo(dT)11 showed these enzymes to be markedly different from mammalian DNA polymerase alpha.

摘要

从 fasciculata 锥虫中部分纯化出了两种类型的 DNA 聚合酶活性。α 型 DNA 聚合酶 A 分子量高，对 N - 乙基马来酰亚胺敏感；而 β 型 DNA 聚合酶 B 分子量低，对 N - 乙基马来酰亚胺有抗性。磷酸纤维素层析显示 DNA 聚合酶 A 活性有多个峰，其特性，如最适 pH、盐敏感性、合成模板 - 引发剂复合物的利用以及对 DNA 聚合酶抑制剂的反应都相似。fasciculata 锥虫 DNA 聚合酶 A 酶对其中一些抑制剂的反应以及聚（rA）X 寡聚（dT）11 的利用表明这些酶与哺乳动物 DNA 聚合酶 α 明显不同。