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[将蝾螈色素上皮移植到无晶状体眼房后细胞增殖的放射自显影研究]

[Radioautographic study of cell proliferation in the pigment epithelium of tritons after transplantation into the cavity of a lens-free eye].

作者信息

Mitashov V I, Grigorian E N

出版信息

Ontogenez. 1984 Jan-Feb;15(1):49-54.

PMID:6700938
Abstract

The proliferative activity of the pigment epithelium cells transplanted in the lens-less eyes was studied in the adult crested newt. The cells of transplanted pigment epithelium incorporated 3H-thymidine injected intraperitoneally. Within 10 days after explantation, the index of labelled nuclei equaled 27.8-34.0% and within 20 days the number of labelled cells doubled. By that time the proliferating transplant cells were depigmented and formed 2-3 rows of cells of retinal rudiment. In response to the removal of lens from the of recipients eyes their regeneration proceeded. Irrespective of participation (dorsal iris) or nonparticipation in lens regeneration (ventral iris), the index of labelled nuclei in these regions of iris had similar values. The eyes of recipients were also characterized by a local proliferation of pigment epithelium cells in the zones of retinal detachment. In these zones the index of labelled nuclei in the pigment epithelium equaled 11.0-31.3%.

摘要

在成年有冠蝾螈中研究了移植到无晶状体眼中的色素上皮细胞的增殖活性。移植的色素上皮细胞摄取了腹腔注射的3H-胸腺嘧啶核苷。在移植后10天内,标记细胞核指数为27.8 - 34.0%,20天内标记细胞数量翻倍。此时,增殖的移植细胞色素脱失,并形成2 - 3排视网膜原基细胞。受体眼的晶状体摘除后,其再生过程开始。无论是否参与晶状体再生(背侧虹膜参与,腹侧虹膜不参与),虹膜这些区域的标记细胞核指数值相似。受体眼还表现为视网膜脱离区域色素上皮细胞的局部增殖。在这些区域,色素上皮中的标记细胞核指数为11.0 - 31.3%。

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