通过流式细胞术证明人血小板上的组胺受体

Demonstration of histamine receptors on human platelets by flow cytometry.

作者信息

Hallberg T, Dohlsten M, Baldetorp B

出版信息

Scand J Haematol. 1984 Feb;32(2):113-8. doi: 10.1111/j.1600-0609.1984.tb02165.x.

DOI:10.1111/j.1600-0609.1984.tb02165.x

Abstract

Fluoresceinated human albumin conjugated with histamine (FHA-HIS) has been used for the demonstration of histamine receptors on human platelets. Such receptors were demonstrated on 40-63% of peripheral blood platelets in 4 healthy donors. The binding of FHA-HIS was inhibited on 35-79% of the platelets by the histamine H1 receptor antagonists diphenhydramine and clemastine. The histamine H2 receptor antagonist cimetidine blocked the FHA-HIS binding on 14-37% of the platelets. It is concluded that histamine H1 as well as H2 receptors occur on human platelets but the receptors are not equally distributed in the platelet population.

摘要

与组胺结合的荧光素化人白蛋白（FHA-HIS）已被用于证明人血小板上的组胺受体。在4名健康供体的外周血血小板中，40%-63%的血小板上显示出此类受体。组胺H1受体拮抗剂苯海拉明和氯马斯汀可抑制35%-79%血小板上FHA-HIS的结合。组胺H2受体拮抗剂西咪替丁可阻断14%-37%血小板上FHA-HIS的结合。结论是人血小板上存在组胺H1和H2受体，但这些受体在血小板群体中的分布并不均匀。