Silove M, Manchester K L
S Afr Med J. 1984 Mar 24;65(12):479-82.
We have compared the effects of acute ketotic diabetes on the capacity of isolated ribosomes to carry out protein synthesis with the effects of the non-ketotic but hyperosmolar state developed in rats as a model of the human condition by the procedure described by Joffe et al. These workers concluded that the hyperosmolar non-ketotic condition was consistent with the liver receiving adequate insulin but with the periphery being insulin-deficient. In support of this hypothesis we find that there is no impairment of hepatic protein synthesis as judged by the activity of isolated ribosomes, nor of the proportion of ribosomes in polysomes in the hyperosmolar non-ketotic condition, this being in contrast with the ketotic state, where protein synthesis and polysome levels are markedly reduced. However, the hyperosmolar non-ketotic condition was characterized by a reduced capacity for protein synthesis by ribosomes isolated from kidney and cardiac and skeletal muscle.
我们将急性酮症糖尿病对分离核糖体进行蛋白质合成能力的影响，与通过乔菲等人描述的方法在大鼠中建立的非酮症但高渗状态（作为人类疾病模型）的影响进行了比较。这些研究人员得出结论，高渗非酮症状态与肝脏接受足够胰岛素但外周胰岛素缺乏相一致。为支持这一假设，我们发现，通过分离核糖体的活性判断，高渗非酮症状态下肝脏蛋白质合成未受损，多核糖体中核糖体的比例也未受损，这与酮症状态形成对比，在酮症状态下蛋白质合成和多核糖体水平显著降低。然而，高渗非酮症状态的特征是，从肾脏、心脏和骨骼肌分离的核糖体蛋白质合成能力降低。
