乙醛处理后大鼠脑和肝分离完整线粒体的亚细胞醛脱氢酶活性及乙醛氧化作用

Subcellular aldehyde dehydrogenase activity and acetaldehyde oxidation by isolated intact mitochondria of rat brain and liver after acetaldehyde treatment.

作者信息

Shiohara E, Tsukada M, Chiba S, Yamazaki H, Nishiguchi K, Miyamoto R, Nakanishi S

出版信息

Toxicology. 1984 Feb 14;30(1):25-30. doi: 10.1016/0300-483x(84)90059-3.

PMID:6701903

Abstract

The effect of treatment of rats with acetaldehyde on the subcellular NAD+-aldehyde dehydrogenase (EC 1.2.1.3, ALDH) activities and acetaldehyde oxidation by isolated intact mitochondria of the liver and the brain was studied. Inhalation of acetaldehyde caused a significant decrease in the liver mitochondrial low Km-ALDH activity, while brain mitochondrial ALDH activity remained unchanged. Acetaldehyde oxidation by isolated intact liver mitochondria decreased significantly but that by brain mitochondria remained unchanged after acetaldehyde inhalation. These findings raise the possibility that the brain enzyme may be exposed to lower concentration of acetaldehyde than the liver enzyme.

摘要

研究了用乙醛处理大鼠对亚细胞NAD⁺-乙醛脱氢酶（EC 1.2.1.3，ALDH）活性以及肝脏和大脑分离的完整线粒体对乙醛氧化作用的影响。吸入乙醛导致肝脏线粒体低Km-ALDH活性显著降低，而脑线粒体ALDH活性保持不变。吸入乙醛后，分离的完整肝脏线粒体对乙醛的氧化作用显著降低，但脑线粒体对乙醛的氧化作用保持不变。这些发现增加了一种可能性，即脑酶可能比肝酶接触到更低浓度的乙醛。