Vigna S R
Am J Physiol. 1984 Mar;246(3 Pt 1):G296-304. doi: 10.1152/ajpgi.1984.246.3.G296.
Radioimmunoassay, radioreceptor assays, and bioassays were used to demonstrate that chicken brain and antrum extracts contain cholecystokinin (CCK)-like and gastrinlike peptides, respectively. C-terminal-specific radioimmunoassay of partially purified chicken CCK and gastrin gave dilution curves parallel to those of the mammalian peptides. Mouse cerebral cortical and rat pancreatic membrane radioreceptor assays were used to differentiate CCK- from gastrinlike peptides on the basis of the different CCK versus gastrin specificities of the two receptors. Confirmation of the biological activity of chicken brain CCK was obtained by stimulation of amylase secretion from rat pancreatic lobules in vitro. The specificity of this response was demonstrated by the inhibition of chicken CCK-stimulated amylase secretion by the specific CCK receptor antagonist dibutyryl cGMP. Chicken antral gastrin stimulated gastric acid secretion from the rat stomach in vivo. In contrast to previous hypotheses, it is proposed that chickens have significant amounts of an antral gastrinlike peptide and that therefore it is possible that gastrin is involved in the physiological regulation of gastric acid secretion in chickens.
放射免疫分析、放射受体分析和生物分析被用于证明鸡脑提取物和胃窦提取物分别含有胆囊收缩素（CCK）样肽和胃泌素样肽。对部分纯化的鸡CCK和胃泌素进行C末端特异性放射免疫分析，得到的稀释曲线与哺乳动物肽的稀释曲线平行。利用小鼠大脑皮质和大鼠胰腺膜放射受体分析，根据两种受体对CCK与胃泌素的不同特异性，区分CCK样肽和胃泌素样肽。通过体外刺激大鼠胰腺小叶分泌淀粉酶，证实了鸡脑CCK的生物活性。特异性CCK受体拮抗剂二丁酰环鸟苷酸抑制鸡CCK刺激的淀粉酶分泌，证明了这种反应的特异性。鸡胃窦胃泌素在体内刺激大鼠胃分泌胃酸。与先前的假设相反，有人提出鸡含有大量的胃窦胃泌素样肽，因此胃泌素可能参与鸡胃酸分泌的生理调节。
